Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: Molecular docking visualization. ( A ) Molecular docking mode diagram of Laurolitsine with ADRB2; ( B ) Molecular docking mode diagram of Hecogenin with MAPK8; ( C ) Molecular docking mode diagram of Hecogenin with MAPK9; ( D ) Molecular docking mode diagram of Hecogenin with MAPK10.

Article Snippet: The following primary antibodies were used in the study: Collagen I (139 kDa, ab260043) and Collagen III (139 kDa, ab184993) from Abcam Shanghai Trading Co., Ltd. (Shanghai, China); α-SMA (42 kDa, BF9212) from Affinity Biosciences (Changzhou, Jiangsu, China); ADRB2 (46 kDa, HY- P81085 ) from MedChemexpress Co., Ltd. (Monmouth Junction, NJ, USA); p-ADRB2 (46 kDa, AF3117) from Affinity Biosciences (Changzhou, Jiangsu, China); JNK (42, 50 kD, 66210-1-Ig) and p-JNK (42, 50 kDa, 80024-1-RR) from Proteintech Group, Inc. (Wuhan, Hubei, China); c-Jun (43 kDa, A11378) and p-c-Jun (43 kDa, 3270T) from ABclonal Biotechnology Co., Ltd. (Wuhan, Hubei, China) and Cell Signaling Technology, Inc. (Danvers, MA, USA), respectively; IL-6 (30 kDa, BA4339) from BOSTER Biological Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: Protein expression of Collagen I, Collagen III and α-SMA in CFs of rats in each group. ( A ) Electrophoretic bands of Collagen I, Collagen III, α-SMA, p-ADRB2, ADRB2, p-JNK, JNK, p-c-Jun, c-Jun, and IL-6 protein expression; ( B ) Relative protein expression level of Collagen I; ( C ) Relative protein expression level of Collagen III; ( D ) Relative protein expression level of α-SMA. Compared to the control group, #### p < 0.0001. Compared to the ISO model group, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following primary antibodies were used in the study: Collagen I (139 kDa, ab260043) and Collagen III (139 kDa, ab184993) from Abcam Shanghai Trading Co., Ltd. (Shanghai, China); α-SMA (42 kDa, BF9212) from Affinity Biosciences (Changzhou, Jiangsu, China); ADRB2 (46 kDa, HY- P81085 ) from MedChemexpress Co., Ltd. (Monmouth Junction, NJ, USA); p-ADRB2 (46 kDa, AF3117) from Affinity Biosciences (Changzhou, Jiangsu, China); JNK (42, 50 kD, 66210-1-Ig) and p-JNK (42, 50 kDa, 80024-1-RR) from Proteintech Group, Inc. (Wuhan, Hubei, China); c-Jun (43 kDa, A11378) and p-c-Jun (43 kDa, 3270T) from ABclonal Biotechnology Co., Ltd. (Wuhan, Hubei, China) and Cell Signaling Technology, Inc. (Danvers, MA, USA), respectively; IL-6 (30 kDa, BA4339) from BOSTER Biological Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Control

Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: Protein expression levels of ADRB2/JNK/c-Jun signaling pathway-related proteins and IL-6 in CFs of each group. ( A ) Relative expression levels of p-ADRB2; ( B ) Relative expression levels of p-JNK; ( C ) Relative expression levels of p-c-Jun; ( D ) Relative expression levels of IL-6. Compared with the Control group, #### p < 0.001. Compared with the ISO Model group, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following primary antibodies were used in the study: Collagen I (139 kDa, ab260043) and Collagen III (139 kDa, ab184993) from Abcam Shanghai Trading Co., Ltd. (Shanghai, China); α-SMA (42 kDa, BF9212) from Affinity Biosciences (Changzhou, Jiangsu, China); ADRB2 (46 kDa, HY- P81085 ) from MedChemexpress Co., Ltd. (Monmouth Junction, NJ, USA); p-ADRB2 (46 kDa, AF3117) from Affinity Biosciences (Changzhou, Jiangsu, China); JNK (42, 50 kD, 66210-1-Ig) and p-JNK (42, 50 kDa, 80024-1-RR) from Proteintech Group, Inc. (Wuhan, Hubei, China); c-Jun (43 kDa, A11378) and p-c-Jun (43 kDa, 3270T) from ABclonal Biotechnology Co., Ltd. (Wuhan, Hubei, China) and Cell Signaling Technology, Inc. (Danvers, MA, USA), respectively; IL-6 (30 kDa, BA4339) from BOSTER Biological Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Control

Journal: Current Issues in Molecular Biology

Article Title: Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays

doi: 10.3390/cimb47100798

Figure Lengend Snippet: The schematic diagram illustrates the molecular mechanism through which MG suppresses IL-6 production in cardiac fibroblasts (CFs): in the isoproterenol (ISO)-induced CFs transdifferentiation model, MG-EWE and its active components Laurolitsine and Hecogenin can inhibit the abnormal activation of the ADRB2/JNK/c-Jun signaling pathway. When ISO binds to the ADRB2 receptor on the cell membrane surface of CFs, it initiates a downstream signaling cascade. Activation of this receptor leads to the phosphorylation of key signaling molecules, including JNK1/2/3 and c-Jun, thereby activating the JNK/c-Jun signaling pathway. The activated JNK1/2/3 translocates from the cytoplasm to the nucleus and further phosphorylates the transcription factor c-Jun within the nucleus, enhancing its transcriptional activity. This ultimately promotes the transcription of the IL-6 gene and increases its protein expression. Laurolitsine functions by inhibiting the activation of ADRB2 on the CFs cell membrane surface, while Hecogenin inhibits the phosphorylation of JNK, thereby blocking the subsequent phosphorylation of c-Jun. Together, these actions result in the joint inhibition of IL-6 production.

Article Snippet: The following primary antibodies were used in the study: Collagen I (139 kDa, ab260043) and Collagen III (139 kDa, ab184993) from Abcam Shanghai Trading Co., Ltd. (Shanghai, China); α-SMA (42 kDa, BF9212) from Affinity Biosciences (Changzhou, Jiangsu, China); ADRB2 (46 kDa, HY- P81085 ) from MedChemexpress Co., Ltd. (Monmouth Junction, NJ, USA); p-ADRB2 (46 kDa, AF3117) from Affinity Biosciences (Changzhou, Jiangsu, China); JNK (42, 50 kD, 66210-1-Ig) and p-JNK (42, 50 kDa, 80024-1-RR) from Proteintech Group, Inc. (Wuhan, Hubei, China); c-Jun (43 kDa, A11378) and p-c-Jun (43 kDa, 3270T) from ABclonal Biotechnology Co., Ltd. (Wuhan, Hubei, China) and Cell Signaling Technology, Inc. (Danvers, MA, USA), respectively; IL-6 (30 kDa, BA4339) from BOSTER Biological Technology Co., Ltd. (Wuhan, Hubei, China).

Techniques: Activation Assay, Membrane, Phospho-proteomics, Activity Assay, Expressing, Blocking Assay, Inhibition